Discrimination of Thermophilic Proteins and Non-thermophilic Proteins Using Feature Dimension Reduction

Proteins from thermophilic microorganisms.

INTRODUCTION .......... ....................................... 320 THE THERMOPHILIC MICROORGANISMS .......... ......................... 321 POSSIBLE MECHANISMS OF SURVIVAL .......... .......................... 322 Lipids ................................................. 322 Rapid Resynthesis ............ ..................................... 322 Thermally Stable Macromolecules ...................

Amino acid coupling patterns in thermophilic proteins.

Structural analysis is useful in elucidating structural features responsible for enhanced thermal stability of proteins. However, due to the rapid increase of sequenced genomic data, there are far more protein sequences than the corresponding three-dimensional (3D) structures. The usual sequence-based amino acid composition analysis provides useful but simplified clues about the amino acid type...

Lessons in stability from thermophilic proteins.

Studies that compare proteins from thermophilic and mesophilic organisms can provide insights into ability of thermophiles to function at their high habitat temperatures and may provide clues that enable us to better define the forces that stabilize all proteins. Most of the comparative studies have focused on thermal stability and show, as expected, that thermophilic proteins have higher Tm va...

Sequence Based Prediction of Protein Mutant Stability and Discrimination of Thermophilic Proteins

How do thermophilic proteins and proteomes withstand high temperature?

We attempt to understand the origin of enhanced stability in thermophilic proteins by analyzing thermodynamic data for 116 proteins, the largest data set achieved to date. We compute changes in entropy and enthalpy at the convergence temperature where different driving forces are maximally decoupled, in contrast to the majority of previous studies that were performed at the melting temperature....